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Teeth and mouth are the supportive organs to enable us in doing all the functions of the body by consuming. So, cleanliness of the oral areas is important for the healthy and nourished body which is devoid of microorganisms. Herbs have been in the investigations for the antibacterial and antiviral properties along with the history of the medical systems. They were effective and safer relative to the available synthetic drugs. Three formulations of gargle were prepared using Extract of Neem, Aloe Gel and clove oil which were named as GNF, GAF and GCF. The physical parameters were estimated, such as pH and one formulation failed in the stability study. The prepared GNF and GAF showed better antibacterial activity against the bacteria extracted by swabbing the mouth and also the three strains like E coil, Streptomyces and staphylococcus.