Protein –protein docking studies on diabetes mellitus using insilico methods

Abstract

Concurrent with the spread of the western lifestyle, the prevalence of all types of diabetes is on the rise in the world's population. The number of diabetics is increasing by 4-5% per year. It was long believed that the effects of insulin are mediated by a unique insulin receptor. There is at present no available drug other than insulin itself to activate directly insulin receptors. It is hoped that these expanded choices will provide the tools necessary for a more efficient management of diabetes and it’s prevalent. In this study we are focused to find out the binding efficiency between the bovine and porcine insulin with the insulin receptor. In this project first the number of binding regions in the insulin receptor was found using CASTP server. From the CASTP analysis we found 110 pockets were present in the insulin receptor structure. Then the analysis was carried out to find out the minimum energy required for binding the protein molecules like bovine and porcine insulin with the insulin receptor using HEX software. The analysis results show that the bovine insulin needs minimum energy of (-334.84) when compared with the porcine insulin binding energy of (-201.13) for producing maximum effect. We also considered the binding energy of these two proteins. The bovine insulin requires the binding energy of -5.62 and the porcine insulin requires 2.69.From the above analysis we concluded that the bovine insulin possess maximum binding capacity with minimum of energy requirements than the porcine insulin.