Antimicrobial studies of Pithecellobium dulce (Roxb.)Benth. against Gastroenteritis causing bacteria and their drug docking studies using Insilico Protocols

Abstract

Gastroenteritis is caused when bacteria gains entry into your gut. This leads to inflammation in your stomach and intestines. It is otherwise called as “Stomach Flu’’. The symptoms are vomiting, severe abdominal cramps, and diarrhea. Some people call this infection as “food poisoning”. Gastrointestinal disturbances resulting from the ingestion can have a variety of causes, e.g., overeating, allergies, nutritional deficiencies, actual poisoning by chemicals, toxic plants or animals, toxins produced by bacteria, infestation by animal parasites and infection by microorganisms. Medicinal plants play a vital role in the treatment of diseases and are an important source of all the pharmaceuticals in the world . Bacterial gastroenteritis usually last for two to three days. In certain cases, these infections can last for more than a week and could be harmful if left untreated. Pithecellobium dulce is a well-known Indian medicinal plant. The stem is used for treating dysentery, leaves for intestinal disorders and seeds for ulcers . The literature review encompasses information on systematic position, ethnobotanical data, folklore claims and its related taxa, biological studies, phytochemistry, antimicrobial studies and Drug Docking studies of Pithecellobium dulce prompted the exploration of the aforesaid areas of studies . The present investigation of the plant, Pithecellobium dulce is aimed to standardize the botanical characters of the plant by screening of the extracts for potential antimicrobial activities. The objectives of the present research investigation is also to find out the molecular binding affinities of the extract plant chemical compound and human pathogens causing gastroenteritis infection (klebshiella oxytoca, E-coli, Pseudomonas aureginosa, Enterobacter sp, Citrobacter Freunboii, klebshiella pneumonia, Proteus mirabilis, Morgonella morgonnoi, Enterobacter cloacae and Proteus vulgaris) using insilico protocols. The bark part of the Pithecellobium dulce was used for its antibacterial activity. Antimicrobial susceptibility testing was done to determine the antimicrobial activities of plant extract against pathogenic bacteria isolated from the stool culture sample. Drug Docking studies were done on isolated bacteria by identifying their multi drug efflux system protein and by using insilico protocols. Thus, we identified that the chemical compounds in the plant products are best agents to reduce the problems associated to gastroenteritis infections. The identified compounds have antioxidant activity whereas these results clearly show that the compounds also have anti-bacterial properties based on in silico docking studies.