Isolation of Alcoholic Extract of Cissus Quadrangularis and Evaluation of InVitro Anthelmintic Activity

Abstract

The aim of the present study was the collection and isolation of plant extract of Cissus quadrangularis Linn root to evaluate the in vitro anthelmintic activity on earthworm Pheretima posthuma (Annelida). For the above reason alcoholic and aqueous extract of Cissus quadrangularis Linn root was prepared and the in vitro anthelmintic activity was evaluated. Both alcoholic and aqueous extract of Cissus quadrangularis Linn root were subjected to qualitative phytochemical tests for different constituents and it was confirmed that the plant extract contains constituents such as Phenolic compounds, tannins, proteins, saponins, steroids, carbohydrates, glycosides and triterpenoids etc. The alcoholic extract of Cissus quadrangularis Linn root at 100 mg/ml, caused paralysis and death at 17.00 and 30.67 minutes respectively, while, 200 mg/ml, caused paralysis and death at 8.33 and 18.50 minutes respectively against earthworm Pheretima posthuma when compared with the reference drug piperazine citrate which showed the same at 19.26 and 63.25 minutes, respectively.