DEVELOPMENT AND VALIDATION OF NEW ANALYTICAL METHOD FOR THE ESTIMATION OF LAMIVUDINE IN BULK AND TABLET DOSAGE FORM BY UV SPECTROPHOTOMETRY

Abstract

Lamivudine (LMV), nucleoside reverse transcriptase inhibitorsNRTI, used as antiretroviral agents in the treatment of HIV-1 infection. An UV spectrophotometric method for the quantitative determination of Lamivudine in bulk and tablets was developed in present work. Distilled water : Ethanol (90:10) was used as solvent. The wavelengths selected for the absorption correlation method was 271 nm. The method was found to be linear between the ranges of 2.5-20 μg/ml. The mean percentage recovery was found in the range of 99.91%-100.79% at three different levels of standard additions. The precision (intra-day, inter-day) of method were found within limits (RSD <2%). Thus the proposed method was simple, precise, economic, rapid and accurate and can be successfully applied for the determination of Lamivudine in bulk and its tablet dosage form.