PHYTOCHEMICAL SCREENING AND EVALUATION OF ETHANOLIC EXTRACT OF COUROUPITA GUIANENSIS FLOWER ON SERUM CARDIAC MARKERS LIKE CK-MB AND LDH

Abstract

Couroupita guianensis has showed a broad spectrum of antibacterial and antifungal activites. The fruit pulp, bark and flowers are used for medicinal applications and have antimicrobial and fungal activity. In the present study an attempt was made to evaluate cardiac protective activity of ethanolic extract of Couroupita guianensis flowers on isoproterenol induced myocardial infarction (MI). Phyto chemical screening revealed the presence of carbohydrates, alkaloids, phenols, tannins, saponins, glycosides, flavonoids, terpenes. Results revealed that there was significant (p < 0.001) increase of serum CK-MB (Creatinine kinase – Muscle and Brain) and LDH (Lactate Dehydrogenase) levels in ischemic control when compared with normal control. There was significant ( p < 0.001) decrease in serum levels of CK- MB and LDH in groups treated with Couroupita guianensis at doses of 100 and 200 mg/kg, p.o, when compared with ischemic control group. Results suggested that reduced myocardial tissue damage was observed in animals treated with high dose of Couroupita guianensis compared with low dose extract.