A NEW RP-HPLC METHOD FOR THE SIMULTANEOUS ASSAY OF SOFOSBUVIR AND VELPATASVIR IN COMBINED DOSAGE FORM

Abstract

A new RP-HPLC method was developed for the simultaneous assay of sofosbuvir and velpatasvir in combined dosage form, using Inertsil ODS column (Make: 150 mmx4.6 mm I.D; particle size 5µm and a mobile phase composed of TFA- Buffer(pH -2.0), Acetonitrile and Methanol (30:50:20% v/v/v) at a flow rate of 1.0mL/min. The retention times of sofosbuvir and velpatasvir were found to be 7.668 and 3.872 min, respectively. Linearity was established for sofosbuvir and velpatasvir in the concentration ranges of 40-120ìg/ml and 1030ìg/ml, respectively. Regression analysis showed a correlation coefficient of greater than 0.999 for sofosbuvir and velpatasvir. The percentage recoveries of sofosbuvir and velpatasvir were found to be in the range of 99.2 to 100.9% and 98.40 to 100.9% respectively. This proposed RP-HPLC method can be successfully employed for simultaneous quantitative analysis of sofosbuvir and velpatasvir in various combined formulations.