Evaluation of Free Radical Scavenging Potential of methanolic extract of Butea Frondosa Leaves

Abstract

The methanolic extract of Buteafrondosa leaves was screened for invitro antioxidant activities by using different in vitro scavenging models .Safer antioxidants suitable for long term use are needed to prevent or stop the progres- sion of free radical mediated disorders such as arthritis, hemorrhagic shock, atherosclerosis, diabetes, hepatic injury, aging, neurodegenerative diseases and carcinogenesis. Antioxidant activity of methanolic extract of Butea- frondosa leaves were assessed using DPPH, hydroxyl, hydrogen peroxide ,nitric oxide in vitro scavenging mod- els.The extract was also studied for metal chelating activity , reducing power , total phenolic compounds and assay on lipid peroxidation induced by Fe²⁺-ascorbate system using rat liver homogenate. The methanolic extract of Bu- teafrondosa leaves exhibited significant scavenging activity in DPPH, hydroxyl, hydrogen peroxide ,nitric oxide in vitro scavenging models and also showed significant metal chelating activity.The reducing power of the extract increased in dose dependant manner. The extract showed marked protection in lipid peroxidation induced by Fe²⁺-ascorbate system using rat liver homogenate. The methanolic extract of Buteafrondosa leaves shows signifi- cant antioxidant activity in different in vitro models. Thus augmenting the wide use of plant in the indigenous sys- tem of medicine for free radical mediated disease.