In vitro anti microbial activity of plant extracts by turbidity method

Abstract

Plants are rich in a wide variety of secondary metabolites such as tannins, terpenoids, alkaloids, and flavanoids which have been found in vitro to have anti microbial properties. The role of anti microbial agents is mainly shift the balance in favour of the host. Our study was to screen the leaf extracts of Moringa oleifera, Withania somnife- ra, Citrullus colocynthis, Salvadora oleiodes, Swerita chirata. These plants were collected and aqueous and alcohol- ic extracts were prepared by decoction and hot percolation process. Microbes for study are staphylococcus, E.coli and Aspergillus species. They were isolated from different sources and identified by morphological and chemical tests. Different dilutions of the test drugs were prepared with saline and by using turbidity method MIC was found and anti fungal activity by spore germination method. Alcoholic extracts preparations of all the plant extracts dis- played higher antibacterial than the aqueous extract preparations. Withania somnifera had highest and strongest anti staphylococcus activity. Citrullus colocynthis showed highest activity against E.coli. From all of the plant ex- tract preparation only alcoholic extract of Salvadora oleoides showed anti fungal activity.