Evaluation of phytochemical screening and analgesic activity of polyherbal formulation on mice

Abstract

Analgesic activity for polyherbal formulation (Mixture of 21 herbs) was evaluated by using hot plate method. Sus- pension of polyherb was prepared by using tween 80. 2 % crude drugs which is present in the form of suspension was administered through oral route at a dose of 100 mg/kg, 300 mg/kg and 500 mg/kg in mice weighing between 25-30 g using oral gavage and evaluated for analgesic activity at different time intervals such as 0, 15, 30, 60 and 90 minutes against standard (Tramadol Hydrochloride 22.8 mg/kg i.p) and control group, P< 0.05 was considered as significant. At a dose 100 mg/kg polyherbal formulation had shown significant activity 12.12 ±0.611 (P < 0.001), 300 mg/kg had shown significant activity at 30, 60 and 90 min time interval and 500 mg/kg had shown significant activity at 15min, 30min, 60min and 90 min (P<0.001).