Antioxidant and anti-inflammatory activity of methanolic, chloroform and ethyl acetate extracts of leaves Tetraclinis articulata (Vahl) Masters Algerian

Abstract

In this study, methanolic, chloroform and acetate ethyl crudes extracts of leaves Tetraclinis articulata (Vahl) Masters obtained by cold maceration and heat maceration were tested to evaluate their antioxidant activity using DPPH method and reducing power and their anti-inflammatory activity using lipooxygenase and xanthine Oxidase inhibition assays. The results show that the methanolic extract obtained by soxhlet showed a strong potential antioxidant with IC 50 (28.55 ± 0.83 mg / ml) higher than that of α-tocopherol (33.49 ± 1.94 mg / ml) and ascorbic acid (31.48 ± 1.37 mg / ml). At 0,5mg/ml, the methanolic crude extract (MeOHc) had the highest ability to reduce Fe (III) and was not significantly different from α-tocopherol (P > 0.05). The absorbance of MeOHc and α- tocopherol were 0.35nm and 0.36nm respectively. At 2mg/ml were 0, 75nm and 0, 77nm respectively and it is also not significantly different (P > 0.05). For lipooxygenase, this extract has a strongest inhibition with 87.25 ±0.45% which is higher than NDGA that is used as control compounds at 50μg/ml and gave an inhibition of 80.34±0.72% and for xanthine Oxidase, has 60.32±0.66%. This same extract had height polyphenol content with 156, 63±0,79mg EAG / g extract.