Cytotoxic and antiproliferative effect of separation methanol extract from stem bark of red mangrove (Bruguiera gymnorrhiza) on hela cells

Abstract

Bruguiera gymnorhiza is one of mangroves that have not been widely studied as an potential anticancer. Previous studies demonstrated that methanol extract from the bark of B. gymnorrhiza has cytotoxic properties against HeLa cancer cells. The purpose of this research is to fractionate the methanol extract with solid liquid partitioning method that is by soaking methanol extracts in n-hexane, chloroform and ethylacetate solutions. Each fraction was tested for it’s cytotoxic activity against HeLa tumor cells with MTT method and continued with antiproliferative and apoptosis induction test. The results showed that the chloroform fraction had the highest activity with L_C50 of 134 µg/mL followed by the fraction of n-hexane with LC₅₀ of 253.3 µg/mLl, ethylacetate fraction of 409.3 µg / ml and the methanol fraction (residue) of 653.3 µg /mL . The antiproliferative test results showed that the fraction can inhibit cell division and cell death.