Effect of different kind of sugars on in vitro production of stigmasterol in Tylophora indica Callus

Abstract

Production of secondary metabolites by cultured cells provides a particularly important benefit to manipulate and improve the production of desired compounds; thus biotechnological approaches to increase the concentrations of the metabolites are discussed. This research paper highlighted the enhancement of stigmasterol, a precursor of hormones in vitro tissue culture of Tylophora indica.In the present study, the effect of different kinds of sugars (Mannitol, Galactose, Fructose, Glucose, Sucrose, Sorbitol, Xylose) in 30g/L concentration separately with Zenk basal medium were examine for the production of stigmasterol in the tissue culture of Tylophora indica. The initia- tion and maintenance of callus was carried out on MS basal media supplemented with 0.2ppm of BAP and 2ppm of NAA, by leaf explants, while Zenk medium was used as a production medium. The sucrose has given the best results in four weeks old callus, which was highest among all test samples used (0.025%) and shown highly signifi- cant increase which was ten times higher than control (0.009%).This protocol for stigmasterol enhancement in Tylophora indica was not documented till date. Statistical data showed that the results were significant (P value

>0.01).