Molecular mechanism of interaction between human sweet taste receptors and antidiabetic agents of Gymnema sylvestre through docking studies

Abstract

Diabetes mellitus is a group of metabolic disorder characterized by hyperglycemia resulting from defects in insulin secretion, insulin action or both. It is classified into two types, type1 and type 2. In this study, sequence analysis of taste receptors and docking studies with antidiabetic agents of Gymnema sylvestre had been carried out. The ac- tive components gurmarin and gymnemic acid of Gymnema sylvestre inhibit the sweet taste response which are going to act as antidiabetic agents. The results showed that the gurmarin and gymnemic acid showed antidiabetic activity. By using bioinformatics tools such as ExPASy translate tool, tertiary structure prediction and docking, the interaction of these active components had been carried out. The results of the present study, first time identified the binding sites of gurmarin and gymnemic acid in both Taste type 1 Receptor 2 and Taste type 1 Receptor 3 (T1R2/T1R3) receptors.