Experimental researches on preclinical toxicity studies of ethanol leaf extract of Cordia sebestena

Abstract

The acute and sub-acute toxicities of the ethanol leaf extract of Cordia sebestena was investigated. For the acute toxicity study, 50-5000 mg/kg of the ethanol leaf extract were administered to rats and obvious toxic symptoms and mortality 24 hours post-administration of the extract were determined. The median lethal dose (LD₅₀) of the extract was determined. In the sub-acute study, 200-400 mg/kg of the extract were administered daily for 14 days. The food and water consumption, body weight changes, as well as heamatological and biochemical parameters were determined periodically. The estimated LD₅₀ of the extract was 4000 mg/kg. There was no mortality during the period of study. The effects on haemoglobin concentration, PCV, RBC and WBC counts were non significant. The extract caused non significant levels in serum liver enzymes, AST, ALP and ALT. Histopathological report also normal which supported the non toxic effect of extract. These results suggest that the leaf extract of Cordia sebes- tena is safe for the treatment of Diabetes mellitus.