Evaluation of Acute and Sub-Chronic Toxicities of the topical extract of Mangifriaindica Seeds on Wistar albino Rats

Abstract

Acute toxicity studies in animals are usually necessary for any pharmaceutical intended for human use. The infor- mation obtained from these studies is useful in choosing doses for repeat-dose studies, providing preliminary identification of target organs of toxicity, and, occasionally, revealing delayed toxicity. Acute toxicity studies may also aid in the selection of starting doses for Phase 1 human studies, and provide information relevant to acute overdosing in humans. The present study was designed to investigate the acute and sub-chronic toxicities of the topical extract of mangifriaindica seed on Wistar albino rats. Toxicity study was carried out by using three differ- ent doses like 1, 2 and 4 mg/kg/day. Several parameters like aspartate transaminase, alanine transaminase, alka- line phosphatase, total protein, albumin, total bilirubin, direct bilirubin, urea, hemoglobin, red blood cell, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin concentration, white blood cell, lympho- cytes, granulocytes and histopathology of liver, kidney, heart and spleen. The extract in all the doses produced significant protection and offered the therapeutic profile related to Mangiferaindica extract.