A review on role of low pressure liquid chromatography in medical research

Abstract

Chromatography is a non-destructive technique used for separating a mixture of compounds into individual com- ponents using a stationery phase and mobile phase Chromatography is used routinely in almost every laboratory for a large number of tasks. The chromatographic techniques are sophisticated among the applied physical chemi- stries chromatographic applications in biochemistry and genetics. Both high pressure and low pressure techniques are have a wide variety of applications in medical sciences. But for HPLC, the limiting factor for many years has been the chromatographic media, because the support matrix must be rigid enough to withstand the applied pressure and still maintain the desired chromatographic qualities. Also the costs of equipment for high pressure techniques are relatively high when compared to low pressure techniques. Hence this leads to development of low pressure liquid chromatographic techniques. These techniques include ion exchange, size exclusion, affinity and hydrophobic interaction chromatography which are used in purification of proteins, antibodies and oligonuc- leotides and many more fields.