SPECTROPHOTOMETRIC QUANTITATION OF SULFAMETHOXAZOLE IN BULK DRUG AND PHARMACEUTICAL FORMULATION USING MULTIVARIATE TECHNIQUE

Abstract

A sensitive and accurate UV spectrophotometric method with multivariate calibration technique for the determination of sulfamethoxazole in bulk and pharmaceutical formulation has been described. This technique is based on the use of the linear regression equations by using relationship between concentration and absorbance at five different wavelengths. The results were treated statistically and were found highly accurate, precise and reproducible. The method is accurate, precise (% recovery r = ) and linear within the range μg/ml. There was no interference from the excipients. This statistical approach gives optimum results for the eliminating fluctuations coming from instrumental or experimental conditions.