GOOD LABORATORY PRACTICES FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC): AN OVERVIEW

Abstract

High performance liquid chromatography is now one of the most powerful tools in analytical chemistry. It has the ability to separate, identify, and quantitate the compounds that are present in any sample that can be dissolved in a liquid. Today, compounds in trace concentrations as low as parts per trillion [ppt] may easily be identified. HPLC can be, and has been, applied to just about any sample, such as pharmaceuticals, food, nutraceuticals, cosmetics, environmental matrices, forensic samples, and industrial chemicals. Nowadays, GLPs are solid standards for government registration and regulatory research facilities. Outside the U.S., OECD GLP is more familiar and has been applied in some other countries. It is important that not only proper science must be used in the scientific research but also quality assurance systems should be implemented while we attempt to produce reliable and reproducible data in regulatory research because all of these activities have great impacts on toxicology and the environment. This article discussed the Good Laboratory practices for High performance Liquid Chromatography for precise results and long life of the instruments and its consumable accessories.