Spectrophotometric estimation of naftopidil in bulk and dosage form

Abstract

A simple, sensitive, accurate, precise, reproducible and inexpensive UV Spectrophotometric method has been developed and subsequently validated for the determination of Naftopidil in bulk and its pharmaceutical formulation. Methanol was used as a solvent in the present investigation. The UV spectrum was scanned between 200 to 400 nm and 232 nm was selected as maximum wavelength for absorption. Beer’s law was obeyed in the concentration range of 2-10 µg/ml with correlation coefficient r = 0.9994.The percent recoveries of naftopidil were found to be 99.75 to 100.293.The intra and inter day precision percent relative standard deviation values in the range of 0.41 to 0.86 and 0.33 to 0.74.The LOD and LOQ were found to be 0.089 µg/ml and 0.27197 µg/ml. The method has been successfully utilized to determine the naftopidil in tablets and can be extended for routine analysis in bulk drugs. Results of the analysis were validated as per ICH guidelines.