Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Telmisartan and Ramipril in Tablet Dosage Forms

Abstract

The present research work deals with the development of RP-HPLC method for the determination of Telmisartan and Ramipril in bulk and in formulation using UV detector. Selected mobile phase was a combination of Acetonitrile: Buffer (0.01M Potassium dihydrogen phosphate) 70: 30 pH 3.4 (adjusted with Orthophosphoric acid) and the wavelength selected was 221nm. The flow rate was kept at 2.0 ml/min, and the injection volume was 20μl. The sepration was performed at ambient temperature. Retention time of Telmisartan and Ramipril was found to be 4.90 and 6.14 minutes respectively. Telmisartan and Ramipril which were found to be linear in the range of 40-240 μg/ml and 10-60 μg/ml respectively. The correlation co-efficient of Telmisartan was found to be 1 and the correlation co-efficient of Ramipril was found to be 1. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 98.92-100.02 for Telmisartan and 99.93100.96 for Ramipril respectively The system suitability parameters such as theoretical plates and tailing factor were found to be 5708, 1.24 and 2460, 1.2 respectively for Telmisartan and Ramipril. This method was validated according to ICH guidelines.