Method development and validation of simultaneous estimation of levosulpiride and rabeprazole in bulk and pharmaceutical dosage form by RP-HPLC

Abstract

A new simple, precise, accurate and selective RP- HPLC method has been developed and validated for estimation of Levosulpiride and Rabeprazole in pharmaceutical formulation. The detection was carried out at 216nm for both drugs. The retention time for LEVO and RAB were found to be 4.918 min. and 5.873 min. respectively. The method was validated for precision, recovery, robustness, specificity, and detection and quantification limits, in accordance with International Conference on Harmonization guidelines. Linearity was observed in the concentration range from 50% to 150% of nominal concentration of Rabeprazole and Levosulpiride Correlation coefficient was 0.999 for both the drugs .The limit of detection and quantification of LEVO were 0.021 mg/ml and 0.0731 mg/ml respectively. While for RAB it was 0.06 mg/ml and 0.20 mg/ml, respectively. The % recovery was found to be within the limits of the acceptance criteria with average recovery of 101.3% for LEVO and 99.3% for RAB. The % RSD below 2.0 shows the high precision of proposed method.