Development method validation of RP-HPLC method for simultaneous determination of lopinavir and Ritonavir in bulk and formulation dosage

Abstract

A reverse phase high performance liquid chromatography method was developed for the simultaneous determination of in tablet dosage form. The determination was performed by the using of two phases one is stationary phase its a thermo hypresil BDS C18 column having 250x4.6mnx 5micrometer and another mobile phase contain (30:70) volumes of water and acetonitrile was mixed and used as mobile phase adjust P.H 7.9 using sodium dihydrogen dihydrate .the flow rate was 2ml min-1 effuent were monitored at 210nm.The retention time of lopinavir and Ritonavir 8.452 and 10.169 respectively and lopinavir and Ritonavir other replicate standard system suitability parameters are within the limits and uniform .validation parameters those are selectivity linearity (correlation coefficients is 1.0000) ,recovery of lopinavir99.6% and Ritonavir 100.3%as per USP acceptence criteria is 98% and 102% ,precision % RSD is less than 1 and also robustness results were uniform they were no marked changes so method is highly validated it use full for pharmaceutical analysis like quality control ,stability and other studies