A NOVEL RP-HPLC METHOD FOR THE ESTIMATION OF NARATRIPTAN HCL IN BULK AND DOSAGE FORM

Abstract

A new simple, precise, sensitive and validated RP-HPLC was developed for the estimation of Naratriptan Hcl in pharmaceutical dosage form. The chromatographic conditions used for the separation was spursil C18, 250 X 4.6 mm, 5 µm particle size and mobile phase comprised of potassium di hydrogen phosphate buffer pH 7.2 and acetonitrile in the ratio (70:30) v/v. The flow rate was 1 ml/min with detection at 224 nm. The retention time was found to be 3.825 min. The linearity was found to be in the range of 50-150 µg/ml with correlation coefficient of 0.999. The proposed method is accurate with 99.55% - 99.56 % recovery for Naratriptan Hcl and precise (%RSD of system and method precision were 1.55,1.21). The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.04956 and 0.1502 µg/ml respectively. The method was successfully applied to pharmaceutical formulation because no chromatographic interferences from tablet excipients were found.