VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS QUANTITATION OF CEFIXIME AND OFLOXACIN IN BULK DRUG AND IN PHARMACEUTICAL FORMULATION

Abstract

This work is concerned with the simultaneous determination of cefixime and ofloxacin in a bulk drug and pharmaceutical formulations by high performance liquid chromatographic (HPLC) method. Chromatographic separation was achieved on Hypersil BDS column, with Potassium dihydrogen phosphate(PH8.9): acetonitrile (60:40v/v) as mobile phase at flow rate of 1ml/min. Quantification was achieved with PDA detector at 288nm.The retention time for cefixime and Ofloxacin was found to be 4.7 and 6.8mins,respectively. The linearity for both the drugs were observed in the concentration range of 50-150µg/ml with mean accuracies 98-101.The method was successfully applied to pharmaceutical formulation because chromatographic interferences from tablet excipients were found.The method retains its accuracy and precision when the standard addition technique was applied.