ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ZIDOVUDINE, LAMIVUDINE AND NEVIRAPINE TABLETS BY RP-HPLC

Abstract

An accurate, precise and reproducible high performance liquid chromatographic method was developed for the estimation of Zidovudine, Lamivudine andNevirapine in pharmaceutical dosage forms. In this method, Hypersil BDS (Base Deactivated Silica) C18 column (150x4.6mm) with mobile phase consisting of Potassium Dihydrogen Phosphate buffer and methanol in the ratio of 60:40 v/v was used. The flow rate was 0.9ml/min and the detection wavelength was 270nm. The linearity was observed in the concentration range of 50%, 75%, 100%, 125%, 150% for the 3 drugs of Zidovudine, Lamivudine and Nevirapine and the correlation coefficients were 1.000,1.000.1.000 respectively. The proposed method was validated for its linearity, accuracy, precision and robustness. The proposed method is simple, rapid, accurate, precise and reproducible hence can be applied for the routine quality control of analysis of Zidovudine, Lamivudine and Nevirapine in pharmaceutical dosage forms.