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This work has been undertaken for the Screening and isolation of Gelatinase producing strains of Bacteria were carried out from Eleven soil samples, collected from various regions of Bangalore and used to screen for Gelatinase production by using Gelatin agar plate assay. In the present study, an attempt was made to isolate efficient Gelatinase producing bacteria from diverse environmental samples. Different isolates were screened for possessing the ability to produce Gelatinase. About 19 bacterial isolates were found to be promising to produce Gelatinase. The organisms were tested for various biochemical tests, which leads to their identification as Bacillus subtillus, Bacillus sphaericus,Bacill pasteurii and Staphylococcus aureus.
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