RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF LOSARTAN POTASSIUM AND PERINDOPRIL ERBUMINE IN PHARMACEUTICAL DOSAGE FORM

Abstract

A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Losartan Potassium and Perindopril erbumine in pharmaceutical dosage form. The mobile phase consisted of Acetonitrile: pot.dihydrogen phosphate buffer (0.1% Trifluoroacetic acid is used as apeak sharpener) in the ratio of 40:60 delivered at a flow rate of 0.8 mL / min and wavelength of detection at 217 nm. The retention times of were Losartan Potassium and Perindopril erbumine 5.87 min and 3.61 min respectively. The method was validated in terms of linearity, precision, accuracy, limit of detection, limit of quantification. The method was found to be linear in the range of 25-150μg/ml and 1-6 μg/ml for Losartan potassium and Perindopril erbumine respectively. The coefficient of variance for both the drug was more than 0.999.The proposed method can be used for determination of these drugs in combined dosage forms.