OPTIMIZATION OF PHYSICO-CHEMICAL PARAMETERS FOR PIGMENT PRODUCTION IN BACTERIA ISOLATED FROM DIFFERENT EFFLUENT WATER SAMPLES

Abstract

Prodigiosin and Carotenoid (Dihydroxy C50 Carotenoid) producing Serratia marcescens and Micrococcus luteus were isolated from different effluent samples and cultured in Nutrient Agar Medium. In the presnt study conducted maximum pigments (Prodigiosin and Carotenoid) production was observed at pH 7.5, temperatures at which the pigments showed optimum production was at room temperature (25ºC) and 16ºC, respectively, optimum concentration of carbon source was at 0.5% and the concentration of nitrogen source was at 0.5% and 2.0%, respectively. After incubation under optimised conditions, the broth was centrifuged and the supernatant was used as the pigment sample. The samples (pigments) were subjected to Thin Layer Chromatography (TLC), which showed spots on the TLC plate and the Rf value was calculated for different spots produced by the pigments. Serratia marcescens and Micrococcus luteus produced maximum amount of the pigments under these optimised conditions, which could be used to increase the production of Prodigiosin and Carotenoid pigments, respectively.