A NEW VALIDATED RP – HPLC METHOD FOR DETERMINATION OF MILNACIPRAN IN TABLET DOSAGE FORMS

Abstract

A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the estimation of Milnacipran in pharmaceutical dosage forms. The mobile phase consist of buffer (0.02M sodium dihydrogen phosphate, pH-3.6 adjusted with ortho phosphoric acid): acetonitrile in the ratio of 60:40 v/v delivered at a flow rate of 1.0 ml / min and wavelength of detection at 220 nm. The retention time of Milnacipran was 4.22 min. The developed method was validated according to ICH guidelines. The result indicates that the method was found to be simple, rapid, and accurate and can be adopted in routine analysis of Milnacipran in Pharmaceutical dosage forms.