DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF METFORMIN HCL AND GLIMEPIRIDE IN COMBINED TABLET DOSAGE FORM

Abstract

An isocratic, reversed phase-liquid-chromatographic method was developed for the quantitative determination of
Metformin Hcl And Glimepiride in combined-dosage form. A Waters Symmetry Shielde Rp18, (250*4.6*5μ) column with mobile
phase containing pH 9.2 adjusted with ortho phosphoric acid : Methanol in the ratio of (60: 40, v/v) was used. The flow rate was
1.0 mL/min, column temperature was 30°C and effluents were monitored at 285 nm. The retention times of Metformin Hcl And
Glimepiride were 2.344min and 3.725min, respectively. The correlation co-efficient for Metformin Hcl And Glimepiride was
found to be 0.99 and 0.99, respectively. The proposed method was validated with respect to linearity, accuracy, precision,
specificity, and robustness. Recovery of Metformin Hcl And Glimepiride in formulations was found to be in the range of 97-103%
and 97-103% respectively confirms the non-interferences of the excipients in the formulation. Due to its simplicity, rapidness and
high precision. The method was successfully applied to the estimation of Metformin Hcl And Glimepiride in combined dosage
form.