SIMULTANEOUS ESTIMATION OF MUPIROCIN AND MOMETASONE FUROATE IN PHARMACEUTICAL DOSAGE FORM BY Q-ABSORPTION RATIO METHOD

Abstract

A simple, rapid, accurate, precise and economical UV-spectrophotometric method have been developed and validated for simultaneous estimation of Mupirocin and MometasoneFuroate in a Pharmaceutical dosage form. The Q absorbance ratio method, which involves formation of Q-absorbance equation at 226 nm (isoabsorptive point) and also at 220 nm (λmax of Mupirocin).Developed methods were validated according to ICH Q2 (R1) guidelines. The methods were found to be linear between the range of 5 - 25 µg/ml for MometasoneFuroate and Mupirocin. The precision (intra-day, inter-day) of method were found within limits (RSD <2%). Accuracy was determined by recovery studies and showed % recovery between 98 to 102%.