ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OFDICLOFENAC SODIUM AND THIOCOLCHICOSIDEIN TABLET DOSAGE FORM BY USING RP-HPLC

Abstract

An isocratic, reversed phase-liquid-chromatographic method was developed for the quantitative determination of
Diclofenac Sodium and Thiocolchicosidein combined-dosage form. A Waters Symmetry ShieldeRp18, (250*4.6*5μ) column with
mobile phase containing water pH 9.2adjusted withdi- Potassium hydrogen Phosphate: Methanol in the ratio of (60: 40, v/v) was
used. The flow rate was 1.0 mL/min, column temperature was 30°C and effluents were monitored at 223 nm. The retention times
of Diclofenac Sodium and Thiocolchicosidewere 3.229 min and 4.999 min, respectively. The correlation co-efficient for
Diclofenac Sodium and Thiocolchicosidewas found to be 0.99and 0.99, respectively. The proposed method was validated with
respect to linearity, accuracy, precision, specificity, and robustness. Recovery of Diclofenac Sodium and Thiocolchicosidein
formulations was found to be in the range of97-103% and 97-103% respectively confirms the non-interferences of the excipients
in the formulation. Due to its simplicity, rapidness and high precision. The method was successfully applied to the estimation of
Diclofenac Sodium and Thiocolchicosidein combined dosage form.