Development and validation for determination of lisinopril dihydrate in bulk drug and formulation using RP-HPLC method

  • Zahid Zaheer Department of Quality Assurance, Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad – 431001. Maharashtra, India
  • Sarfaraz Khan Department of Quality Assurance, Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad – 431001. Maharashtra, India
  • Mohammad Sadeque Department of Quality Assurance, Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad – 431001. Maharashtra, India
  • Hundekari G. I. Department of Quality Assurance, Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad – 431001. Maharashtra, India
  • Rana Zainuddin Department of Quality Assurance, Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad – 431001. Maharashtra, India
Keywords: Lisinopril, RP-HPLC, Validation

Abstract

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for Lisinopril in bulk drug and formulation. A column having 150 × 4.6 mm in isocratic mode with mobile phase containing acetonitrile: phosphate buffer (70:30; adjusted to pH 3.0) was used. The flow rate was 0.8 ml/min and effluent was monitored at 216 nm. The retention time (min) and linearity range (μg/ml) for Lisinopril was (1.510) and (10-35). The developed method was found to be accurate, precise and selective for determination of Lisinopril in bulk and formulation.

Published
2018-06-25
Section
Original Article