Immunomodulatory effects of sulfated polysaccharides from Sargassum siliquosum J.G Agardh on peripheral blood mononuclear cells

Abstract

Sulfated polysaccharides from brown seaweeds exhibit various biological activities and structural diversity, thus are potential reagents for the development of therapeutic drugs. Sulfated polysaccharides isolated from Sargas- sum siliquosum J. G Agardh were investigated for its immunomodulatory potential using in vitro assays. Estimation of immunomodulatory activity included proliferative effects and modulation on production of IL-1. IL-6, TNF-α and MCP-1 in LPS-induced human peripheral blood mononuclear cells. Extracted water soluble polysaccharides con- taining sulfate were identified by FT-IR and Ashing Acid Digestion Ion chromatography with IR spectra comparable to standard fucoidan and alginate. Sulfated polysaccharides were non-cytotoxic and significantly induced prolife- ration of PBMC’s in a dose-dependent manner. Sargassum siliquosum sulfated polysaccharides reduced the pro- duction of pro-inflammatory cytokines such as IL-1, IL-6, TNF-α and MCP-1 in LPS-induced PBMC. Results here in- dicated that sulfated polysaccharide of S. siliquosum possesses immunomodulatory properties and their use as potential natural reagents for immune regulation should be given priority.