Estimation of Fesoterodine fumarate in tablet dosage forms by a new RP-HPLC method

Abstract

A Simple, Sensitive and specific reverse phase high performance liquid chromatographic method has been developed for the determination of Fesoterodine fumarate in tablet Dosage forms. Chromatographic separation was achieved on a Phenomenex, C18 (250×4.6 mm), 5.0 µm column with a 49.5:0.5:50 mixture of 0.025M ammonium dihydrogen phosphate w/v, triethylamine v/v and acetonitrile v/v, Then adjusted the pH to 7.0 with dilute ortho phosphoric acid as mobile phase, detection was at 225 nm. Response was a linear function of concentration in the range 2-0.01 µg/mL for Fesoterodine fumarate. LOD and LOQ for Fesoterodine fumarate were found 0.01 µg/mL and 0.03 µg/mL. Accuracy (recoveries 90-97%) and reproducibility were found to satisfactory.