VELOPMENT AND VALIDATION OF A RAPID HPLC METHOD FOR DETERMINATION OF DEXAMETHASONE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Abstract

A simple and reliable reversed-phase high performance liquid chromatographic (HPLC) method was developed and validated for dexamethasone. The method was employed on a thermo hypersil BDC column (250 mm × 4.6 mm, 5 μm) at ambient temperature. The mobile phase consisted of buffer(0.1Mpotassium dihydrogen phosphate adjust the PH2.3 with ortho phosphoric acid: methanol (60:40) at a flow rate of 1 ml/min. The detection wavelength was set at 226 nm and 10 μL of sample was injected into the HPLC system. Dexamethasone was used as the internal standard. The retention time for internal standard is 4.189mins. The method was linear and correlation coefficient is found to be1.000 The method presented the requisite accuracy, selectivity, sensitivity and precision and showed good results. The proposed method was successfully used for analysis of the drug