THE RESEARCH EVIDENCE OF ANTIOXIDANT AND ANTI-CANCER ACTIVITY OF GENISTEIN CONTENT IN THE INDONESIAN TRADITIONAL FOOD (ONCOM) ETHANOL EXTRACT

Abstract

Oncom ethanol extracts in our previous study contains geneistein which is a potent flavonoid antioxidants and anti-cancer activity. This study is a series of studies to prove that oncom, a traditional Indonesian food, has antioxidant properties, anti-inflammatory and anti-cancer properties. Anti-inflammatory properties are also our previous report. Phytochemical screening in this study showed that ethanol extract contains only condensed flavonoid. Ethanol extract obtained by soxhletation, evaporation and freeze drying tested the antioxidant activity and anti-cancer in vitro by using a 1.1-diphenyl 2-pikrilhidrazil (DPPH) and Sulforhodamin B against breast cancer cells (MCF-7), respectively. Operating time for the antioxidant reaction was 40-100 minutes. By using vitamin C as a standard, antioxidant IC50 for oncom ethanol extract was 363.8 ppm. It was found that the addition of HCl to the extract prior DPPH reaction would increase the IC50 value significantly. For in-vitro antiproliferation tests, pure genistein, oncom ethanol extract and cisplatin as a standard were applied. The genistein had IC50 = 15.45 ppm against MCF-7 cells. The oncom ethanol extract had IC50 = 15.79 ppm whereas standard cisplatin gave IC50 = 4.80 ppm. These data indicated that genistein in oncom extract may cause its anti-cancer activity