Method development and validation of simultaneous estimation of metformin and pioglitazone in bulk and pharmaceutical formulation by UPLC

Abstract

In this work, a rapid, precise and specific ultra performance liquid chromatography (UPLC) method was developed and validated for the simultaneous determination of Metformin and pioglitazone in bulk and pharmaceutical dosage forms. . The chromatographic separation of the drug components were achieved on Waters Acquity BEH C18, 50×2.1 mm, 1.7 μm UPLC column using 0.2% triethylamine in water : acetonitrile(70:30);pH adjusted with 3.8 with o-phosphoric acid as mobile phase 0.5ml/min and detection wavelength was 243nm. Within a short runtime of 5.0 min. The newly developed UPLC method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, system suitability and selectivity, limit of detection and limit of quantitation.