Main Article Content

Abstract

In this work, a rapid, precise and specific ultra performance liquid chromatography (UPLC) method was developed and validated for the simultaneous determination of Metformin and pioglitazone in bulk and pharmaceutical dosage forms. . The chromatographic separation of the drug components were achieved on Waters Acquity BEH C18, 50×2.1 mm, 1.7 μm UPLC column using 0.2% triethylamine in water : acetonitrile(70:30);pH adjusted with 3.8 with o-phosphoric acid as mobile phase 0.5ml/min and detection wavelength was 243nm. Within a short runtime of 5.0 min. The newly developed UPLC method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, system suitability and selectivity, limit of detection and limit of quantitation.

Keywords

UPLC Simultaneous determination Metformin and pioglitazone Validation

Article Details

How to Cite
B, S. B., Sravani, & Kumar, R. (2012). Method development and validation of simultaneous estimation of metformin and pioglitazone in bulk and pharmaceutical formulation by UPLC . International Research Journal of Pharmaceutical and Applied Sciences, 2(4), 91-96. Retrieved from https://scienztech.org/index.php/irjpas/article/view/358