PRODUCTION OF AMYLASE BY USING BACILLUS LICHENIFORMIS ISOLATED FROM VERMICOMPOST

Abstract

Bacillus licheniformis strain isolated from a vermicompost was tested for its abilities to hydrolyze the structural polysaccharides. The effect of different production parameters such as pH, temperature, carbon source, nitrogen source (Organic and inorganic) incubation time inoculum sizes and surfactants on amylase production by the isolated bacterial strain was studied. The enzyme production was assayed in submerged fermentation (SmF) condition. The maximum amylase production was observed with maltose (200±2.6 U/ml), yeast extract (143±3.6U/ml), ammonium sulphate (65±3.0U/ml), pH 7.0 (233±4.1), temperature 40°C (125±9.1), Tween-80 (145±2.8U/ml), inoculum size level 5% (132±2.0U/ml) and incubation time 48 hours (162.01± 0.56U/ml) in the production medium.