Evaluation of Anti-Inflammatory Activity of Citrullus lanatus Seed Oil by In-vivo and In-vitro Models

Abstract

The present study aimed for in-vivo and in-vitro evaluation of Citrullus lanatus seed oil for anti-inflammatory activity. Citrullus lanatus seed oil is extracted with n-hexane and tested for in-vivo and in-vitro antiinflammatory activity. The oil was screened for in-vivo anti-inflammatory activity by carrageenan-induced paw edema in rat model and In-vitro anti-inflammatory activity by human red blood cell membrane stabilization method. The potency of the oil was compared with standard diclofenac (10 mg/kg). The oil showed significant reduction of edema in carrageenan induced rat paw edema model maximum at 3 hr (percentage reduction in paw volume 44.44%, 55.56% and 63.11% for CLSO(50 mg/kg), CLSO(100mg/kg) and diclofenec(10mg/kg) respectably and CLSO at concentration of 100, 250 and 500 mcg/ml showed 42.35%, 68.48% and 78.50% protection of HRBC in hypotonic solution respectably. All the results were compared with standard diclofenac at 50, 100 and 200 mcg/ml which showed 43.74%, 63.93% and 86.73% protection of HRBC in hypotonic solution respectably.