MODULATION OF INSULIN RECEPTOR SUBSTRATE-1 AND GLUCOSE TRANSPORTER 4 EXPRESSIONS IN MOUSE C2C12 MYOBLAST TREATED WITH CYNANCHUM WILFORDII AND PHLOMIS UMBROSA EXTRACTS

Abstract

Phlomis umbrosa and Cynanchum wilfordii were reported to have several immunogenic properties. Problem statement: Our present study was focused on determining the effect of the water extracts of Phlomis umbrosa (PU) and Cynanchum wilfordii (CW) in enhancing insulin receptor substrate 1(IRS-1), glucose transporter 4(GLUT4) expressions and cell proliferation in C2C12 myoblast. Approach: Mouse C2C12 cells were treated with 100, 250, and 500 ug/ml of each extract and the cell viability was measured by WST assay. The IRS-1 and GLUT4 mRNA and proteins expressions were determined by reverse transcriptase PCR and western blot respectively. Results: PU and CW have no cytotoxic effects on the growth of C2C12 cells. The expressions of IRS-1 and GLUT4 proteins were up-regulated following PU and CW treatment. Moreover, both extracts also increases cellular glucose uptake in vivo. Conclusion: Our finding reveals that the PU and CW stimulates IRS-1 and GLUT4 expressions in C2C12 cells. PU and CW also increased glucose uptake on in vivo model. These results suggest that PU and CW can be potential therapeutic agent for the management of diabetes.