DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF BICALUTAMIDE IN PURE AND PHARMACEUTICAL DOSAGE FORMS

Abstract

A simple, accurate, rapid, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the estimation of bicalutamide in pure and pharmaceutical dosage forms. In this method RP-C18 Innersil ODS column with mobile phase consisting of Sodium dihydrogen phosphate buffer(NaH2 PO4) adjusted pH to 3.5 with Ortho phosphoric acid and acetonitrile in the ratio of 65:35v/v was used. The detection wavelength is 272nm and the flow rate 1.5ml/min. The linearity was found in the range of 50-150µg/ml and shows a correlation coefficient of 0.9997. The proposed method was validated by determining sensitivity, accuracy, precision and linearity. The proposed method is simple, fast, accurate and precise hence can be applied for routine quality control analysis of bicalutamide in pure and pharmaceutical dosage forms