ESTIMATION OF CRUDE AND PURIFIED PROTEIN FROM DE-OILED PUPAE OF ERI SILKWORM, PHILOSAMIA RICINI

Abstract

In the present investigation, crude and purified protein percentage was estimated from de-oiled pupae powder at the different hours(0 hours,72 hours,144 hours,216 hours) of pupae development of Eri silkworm,Philosamia ricini was analysed. It was found that crude protein and protein concentrate was gradually increased from 0 hours to 216 hours both in male and female pupae. However, female pupae exhibited 45.1g of protein concentrate at 216 hours of development when compared to male pupae(39.3g).The presence of high protein concentrate in the pupae may be due to the fact that at the beginning of development the protein was utilized for histolysis and the increase of protein at 216 hours may be due to histogenesis of adult organs hence, the gradual increase of protein was observed from 0 hr-216 hrs.