DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN AND TINIDAZOLE IN COMBINED DOSAGE FORM

Abstract

An isocratic, reversed phase-liquid-chromatographic method was developed for the quantitative determination of Ciprofloxacin and Tinidazole in combined-dosage form. A Inertsil C18 (150*4.6*5µ) column with mobile phase containing water pH 2.4 adjusted with ortho phosphoric acid: aceto nitrile in the ratio of (850: 150, v/v) was used. The flow rate was 1.0 mL/min, column temperature was 30°C and effluents were monitored at 275 nm. The retention times of Ciprofloxacin and Tinidazole were 2.419min and 5.119min, respectively. The correlation co-efficient for ciprofloxacin and tinidazole was found to be 0.99 and 0.99, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. Recovery of Ciprofloxacin and Tinidazole in formulations was found to be in the range of 97-103% and 97-103% respectively confirms the non-interferences of the excipients in the formulation. Due to its simplicity, rapidness and high precision. The method was successfully applied to the estimation of Ciprofloxacin and Tinidazole in combined dosage form.