DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF AZITHROMYCIN AND LEVOFLOXACIN IN COMBINED TABLET DOSAGE FORM

Abstract

An isocratic, reversed phase-liquid-chromatographic method was developed for the quantitative determination of Azithromycin and Levofloxacin in combined-dosage form. A Waters Symmetry Shielde Rp18, (250*4.6*5µ) column with mobile phase containing water pH 9.2 adjusted with di- Potassium hydrogen Phosphate: Methanol in the ratio of (60: 40, v/v) was used. The flow rate was 1.0 mL/min, column temperature was 30°C and effluents were monitored at 285 nm. The retention times of Azithromycin and Levofloxacin were 5.001min and 3.232min, respectively. The correlation co-efficient for Azithromycin and Levofloxacin was found to be 0.99 and 0.99, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. Recovery of Azithromycin and Levofloxacin in formulations was found to be in the range of 97-103% and 97-103% respectively confirms the non-interferences of the excipients in the formulation. Due to its simplicity, rapidness and high precision. The method was successfully applied to the estimation of Azithromycin and Levofloxacin in combined dosage form.