PRODUCTION, CHARACTERIZATION & OPTIMIZATION OF POTENT PROTEASE (SERRATIOPEPTIDASE) FROM SERRATIA MARCESCENS E 15

Abstract

Production, characterization & optimization of serratiopeptidase from Serratia marcescens E 15 was the base for this
study. Serratia marcescens E 15 was allowed to grow in trypticase soy broth. The serratiopeptidase(STP) enzyme activity was
determined according to the gelatin clearing zone. Enzyme assay was carried out &protein content was determined. A standard
serratiopeptidase tablet “Serric-5” (5mg) containing 10000 Units of STP enzyme was used for the assay. Protease assay was
carried out to determine the Units of protease in STP. Optimum conditions for STP production were 37oC temperature, 25hrs.
Incubation period & pH=7.3. The STP enzyme was partially purified by ammonium sulphate precipitation & dialysis. STP
enzyme activity increased with the increase in enzyme concentration.