SEMISYNTHESIS AND BIOLOGICAL EVALUATION OF EURYCOMANONE DERIVATIVES AS NEW ANTIMALARIAL

Abstract

Several new eurycomanone derivatives were synthesized by esterification using isolated eurycomanone and acylating agent. Acylating agents which were used for esterification of isolated eurycomanone using acetylchloride, valerylchloride, butirylchloride, methoxybenzoylchloride and succinicanhydride. Eurycomanone and its derivatives were used for in vitro antimalarial activity test. Testing of in vitro antimalarial activity was conducted by candle jar method. Eurycomanone and its derivatives in six levels concentration (0; 1; 10; 100; 1000; and 10.000 ng/mL) were incubated with Plasmodium falciparum strain 3D7 for 72 hours in CO2 incubator at 370 C. Antimalarial activity of eurycomanone and its derivatives were assessed microscopically after making thin blood smear and staining with Giemsa 10%. The fifty percent of P. falciparum growth inhibitory is determined by linear regression analysis. The result showed that eurycomanone more potent than chloroquine and its derivatives. Monoacyl eurycomanone more active than diacyl and triacyl eurycomanone.