A REVIEW ON HYDROPHILIC INTERACTION CHROMATOGRAPHY- A USEFUL TECHNIQUE

Abstract

Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) is a version of partition chromatography which occupies the opposite end of the partition spectrum from reversed phase liquid chromatography. Both are distinct from normal phase liquid chromatography in that water is part of the mobile phase, and thus not adsorption chromatography. When the mobile phase contains > 60% organic solvent, then hydrophilic interaction becomes significant. The mechanism of HILIC implicates partitioning between a water-enriched layer partially immobilized on the hydrophilic stationary phase and the less polar mobile phase. The purpose of this work is to review the mechanism of behind the hydrophilic interaction chromatography by using the stationary phase and mobile phase for the separation of polar compounds. The working of hydrophilic interaction is explained in this article. The separation mechanisms are given with the intermolecular interactions types. The additives and proper pH is used for the separation of compounds by hydrophilic interaction chromatography. The recent advances related to the hydrophilic interaction chromatography are given this article.