DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ISONIAZID AND RIFAMPICIN IN COMBINED DOSAGE FORM

Abstract

An isocratic, reversed phase-liquid-chromatographic method was developed for the quantitative determination of
Isoniazid and Rifampicin in combined-dosage form. A Inertsil ODS (250*4.6*5μ) column with mobile phase containing water
pH 4.5 adjusted with Sodium di hydrogen phosphate: Acetonitrile in the ratio of (400: 600, v/v) was used. The flow rate was 1.0
mL/min, column temperature was 30°C and effluents were monitored at 274 nm. The retention times of Isoniazid and Rifampicin
were 2.953min and 3.382min, respectively. The correlation co-efficient for Isoniazid and Rifampicin was found to be 0.99 and
0.99, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and
robustness. Recovery of Isoniazid and Rifampicin in formulations was found to be 100% and 100% respectively confirms the noninterferences
of the excipients in the formulation. Due to its simplicity, rapidness and high precision. The method was successfully
applied to the estimation of Isoniazid and Rifampicin in combined dosage form.